Lactate Dehydrogenase
乳酸脫氫酶(LDH或LD)是一個廣泛存在于各種生物體中的酶,包括植物與動物。它存在于四種不同的酶分類中,其中兩種是依賴細胞色素c的酶,它們各自作用于D-乳酸與L-乳酸。LDH存在于身體的許多組織和器官中,包括肌肉、肝臟、心臟、胰腺、腎臟、大腦和血細胞。
可檢測樣本中大鼠的LDH,且與其類似物無明顯交叉反應。
由于實驗操作條件的不同( 如操作者、移液技術、洗板技術和穩定條件等),標準曲線的OD值會有所差異。以下數據和曲線僅供參考,實驗者需根據自己的實驗建立標準曲線。
注意:本圖僅供參考,應以同次試驗標準品所繪標準曲線計算標本含量。
板內變異系數小于10%,板間變異系數小于10%
在選取的健康大鼠血清、血漿中加入3個不同濃度水平的大鼠LDH,計算回收率
分別在選取的4份健康大鼠血清、血漿中加入高濃度大鼠LDH,在標準曲線動力學范圍內進行稀釋,評估線性。
注:ELISA試劑盒檢測范圍等數據,因檢測樣本 的不同而調整,實際數據以隨貨說明書為準。
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