cCAT; AAT; ASAT; SGOT; GOT1; Cysteine transaminase, cytoplasmic; Transaminase A; Aspartate Transaminase 1,Cytoplasmic; Glutamic-Oxaloacetic Transaminase 1,Soluble
天門冬氨酸氨基轉移酶(AST)或天門冬氨酸轉氨酶,又稱AspAT/ASAT/AAT或(血清)谷氨酸草酰乙酸轉氨酶(GOT,SGOT),是一種依賴磷酸吡哆醛(PLP)的轉氨酶(AST催化天門冬氨酸和谷氨酸之間α-氨基的可逆轉移,因此它是氨基酸代謝中的一個重要酶。AST存在于肝臟、心臟、骨骼肌、腎臟、大腦和紅血球中。
可檢測樣本中小鼠的AST,且與其類似物無明顯交叉反應。
由于實驗操作條件的不同( 如操作者、移液技術、洗板技術和穩定條件等),標準曲線的OD值會有所差異。以下數據和曲線僅供參考,實驗者需根據自己的實驗建立標準曲線。
注意:本圖僅供參考,應以同次試驗標準品所繪標準曲線計算標本含量。
板內變異系數小于10%,板間變異系數小于10%
在選取的健康小鼠血清、血漿、細胞培養上清中加入3個不同濃度水平的小鼠AST,計算回收率
分別在選取的4份健康小鼠血清、血漿、細胞培養上清中加入高濃度小鼠AST,在標準曲線動力學范圍內進行稀釋,評估線性。
注:ELISA試劑盒檢測范圍等數據,因檢測樣本 的不同而調整,實際數據以隨貨說明書為準。
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